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The engagement of oral-associated lymphoid tissues during oral versus gastric antigen administration

Journal article
Authors Maria Bankvall
Anna Karin Ostberg
Mats Jontell
Agnes E Wold
Sofia M Östman
Published in Immunology
Volume 149
Issue 1
Pages 98-110
ISSN 0019-2805
Publication year 2016
Published at Institute of Odontology
Institute of Odontology, Section 1
Institute of Biomedicine, Department of Infectious Medicine
Institute of Odontology, Section 3
Pages 98-110
Language en
Links dx.doi.org/10.1111/imm.12633
Keywords antigen administration, cervical lymph nodes, murine model, nose-associated lymphoid tissues, intestinal immune-system, t-cells, mucosal tolerance, sublingual, immunotherapy, immunological-tolerance, mice, suppression, induction, mechanisms, nodes, Immunology
Subject categories Clinical Medicine

Abstract

The role of oral-associated lymphoid tissues during induction of oral tolerance still remains elusive. Therefore, the aim was to compare T-cell activation and induction of tolerance to ovalbumin (OVA) presented through either of two routes; deposited into the oral cavity, or the stomach, thereby bypassing the oral cavity. OVA was administered by the oral or gastric route to BALB/c mice that had received OVA-specific DO11.10+ CD4(+) T cells, stained with CellTrace Violet dye, through intravenous injection. Proliferating OVA-specific T cells were detected in the nose-associated lymphoid tissues (NALT) and the cervical, mesenteric and peripheral lymph nodes at different time-points following OVA exposure. OVA-specific T-cell proliferation was initially observed in the NALT 1hr after oral, but not gastric, administration. However, at day 1, proliferation at this site was also detected after gastric administration and profound proliferation was observed at all sites by day 4. For the oral route the degree of proliferation observed was lower in the peripheral lymph nodes by day 4 compared with the other sites. These results demonstrate a similar activation pattern achieved by the two routes. However, the NALT distinguishes itself as a site of rapid T-cell activation towards fed antigens irrespective of feeding regimen. To evaluate induction of tolerance a semi-effective OVA dose was used, to detect differences in the degree of tolerance achieved. This was performed in a model of OVA-induced airway hypersensitivity. No differences in tolerance induction were observed between the two administration routes.

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