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The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets.

Journal article
Authors Weston B Struwe
Sanjay Agravat
Kiyoko F Aoki-Kinoshita
Matthew P Campbell
Catherine E Costello
Anne Dell
Ten Feizi
Stuart M Haslam
Niclas G. Karlsson
Kay-Hooi Khoo
Daniel Kolarich
Yan Liu
Ryan McBride
Milos V Novotny
Nicolle H Packer
James C Paulson
Erdmann Rapp
Rene Ranzinger
Pauline M Rudd
David F Smith
Michael Tiemeyer
Lance Wells
William S York
Joseph Zaia
Carsten Kettner
Published in Glycobiology
Volume 26
Issue 9
Pages 907-910
ISSN 1460-2423
Publication year 2016
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 907-910
Language en
Links dx.doi.org/10.1093/glycob/cww082
Subject categories Basic Medicine, Biological Sciences

Abstract

The minimum information required for a glycomics experiment (MIRAGE) project was established in 2011 to provide guidelines to aid in data reporting from all types of experiments in glycomics research including mass spectrometry (MS), liquid chromatography, glycan arrays, data handling and sample preparation. MIRAGE is a concerted effort of the wider glycomics community that considers the adaptation of reporting guidelines as an important step towards critical evaluation and dissemination of datasets as well as broadening of experimental techniques worldwide. The MIRAGE Commission published reporting guidelines for MS data and here we outline guidelines for sample preparation. The sample preparation guidelines include all aspects of sample generation, purification and modification from biological and/or synthetic carbohydrate material. The application of MIRAGE sample preparation guidelines will lead to improved recording of experimental protocols and reporting of understandable and reproducible glycomics datasets.

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