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Insulin released from titanium discs with insulin coatings- kinetics and biological activity

Journal article
Authors Behnosh Öhrnell Malekzadeh
Maria Ransjö
Pentti Tengvall
Zivko Mladenović
Anna Westerlund
Published in Journal of Biomedical Materials Research. Part B - Applied biomaterials
Volume 105
Issue 7
Pages 1847-1854
ISSN 1552-4973
Publication year 2017
Published at Institute of Odontology
Institute of Clinical Sciences, Department of Biomaterials
Institute of Odontology, Section 3
Pages 1847-1854
Language en
Links dx.doi.org/10.1002/jbm.b.33717
Keywords Insulin, biomaterial, surface modification, osteoblast, titanium
Subject categories Health Sciences

Abstract

Local administration of insulin from a titanium surface has been demonstrated to increase bone formation in non-diabetic rats. The authors hypothesised that insulin was released from the titanium surface and with preserved biological activity after the release. Thus, in the present in vitro study, human recombinant insulin was immobilized onto titanium discs, and the insulin release kinetics was evaluated using Electro-chemiluminescence immunoassay. Neutral Red uptake assay and a mineralization assay were used to evaluate the biological effects of insulin on human osteoblast-like MG-63 cells. The results confirmed that insulin was released from titanium surfaces during a six-week period. Furthermore, etching the disc prior to insulin coating, thickening of the insulin coating and incubation of the discs in serum-enriched cell culture medium increased the release. Another interesting finding was that longer storage time decreased the release of insulin. The released insulin had retained its biological activity, as demonstrated by the significant increase in cell number and a stimulated mineralization process, upon exposure to released insulin.

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