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Neuropharmacological effects of Phoneutria nigriventer venom on astrocytes

Journal article
Authors C. Raposo
Ulrika Björklund
E. Kalapothakis
Björn Biber
M. A. da Cruz-Hofling
Elisabeth Hansson
Published in Neurochemistry International
Volume 96
Pages 13-23
ISSN 0197-0186
Publication year 2016
Published at Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
Institute of Clinical Sciences, Department of Anesthesiology and Intensive care
Pages 13-23
Language en
Links dx.doi.org/10.1016/j.neuint.2016.04...
Keywords Arthropod venom, Astrocytes, Ca2+ responses, Stress fibers, TLR4, Glutamate, blood-brain-barrier, generates calcium oscillations, spider venom, hippocampal astrocytes, plasma-volume, na+/k+-atpase, nitric-oxide, rats, expression, inhibition, Biochemistry & Molecular Biology, Neurosciences & Neurology
Subject categories Biochemistry and Molecular Biology, Neuroscience, Neurology

Abstract

Bites from genus Phoneutria (Ctenidae, Araneomorpha) are the second most frequent source of spider accidents in Southeast Brazil. Severe envenoming from Phoneutria nigriventer produces vision disturbance, tremor and convulsion, suggesting that the CNS is involved; however, the mechanisms by which P. nigriventer venom (PNV) affects the CNS remain poorly understood. The present study aimed to investigate whether PNV directly impairs astrocytes. Cultured astrocytes were exposed to PNV, and intracellular Ca2+ release and signaling were measured (Fura-2/AM), Na+/K+-ATPase and Toll-like receptor 4 (TLR4) involvement were investigated, actin filaments were stained (Alexa (TM) 488-conjugated phalloidin probe), the G-actin/F-actin ratio was determined, and the expression level of connexin 43 (Cx43) was assessed. Incubation in Ca2+-free buffer did not change the Ca2+ responses. However, pre-incubation in thapsigargin/caffeine completely abolished these responses, suggesting that PNV-evoked Ca2+ transients were from intracellular Ca2+ stores. Pretreatment with a Na+/K+-ATPase antagonist (ouabain) or a TLR4 antagonist (LPS-RS) decreased or increased the Ca2+-evoked transients, respectively. Astrocytes showed altered actin filament structure after PNV exposure. PNV treatment increased the expression levels of Na+/K+-ATPase and Cx43 but decreased those of TLR4. The present results suggest that PNV directly affects astrocytes. Na+/K+-ATPase may thus represent a more specific drug target for controlling the neurotoxicity of PNV. (C) 2016 Elsevier Ltd. All rights reserved.

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