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Nested PCR for detection of HSV-1 in oral mucosa

Journal article
Authors M.M. Jalouli
Jamshid Jalouli
Bengt Hasséus
J. Öhman
J.M. Hirsch
L. Sand
Published in Medicina Oral, Patologia Oral y Cirugia Bucal
Volume 20
Issue 6
Pages e664-e669
ISSN 1698-4447
Publication year 2015
Published at Institute of Odontology
Institute of Odontology, Section 1
Pages e664-e669
Language en
Links dx.doi.org/10.4317/medoral.20630
Keywords HSV-1 , Nested PCR , PCR
Subject categories Dentistry

Abstract

© Medicina Oral S. L. Background: It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive. This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC). Material and Methods: We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples. DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing. Results: HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant. Conclusions: We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues. Thus, no connection between OSCC development and presence of HSV-1 was detected.

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