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Bacteria and Fungi Respond Differently to Multifactorial Climate Change in a Temperate Heathland, Traced with C-13-Glycine and FACE CO2

Journal article
Authors Louise C. Andresen
J. A. J. Dungait
R. Bol
M. B. Selsted
P. Ambus
A. Michelsen
Published in Plos One
Volume 9
Issue 1
ISSN 1932-6203
Publication year 2014
Published at
Language en
Subject categories Climate Research, Microbiology, Terrestrial ecology

Abstract

It is vital to understand responses of soil microorganisms to predicted climate changes, as these directly control soil carbon (C) dynamics. The rate of turnover of soil organic carbon is mediated by soil microorganisms whose activity may be affected by climate change. After one year of multifactorial climate change treatments, at an undisturbed temperate heathland, soil microbial community dynamics were investigated by injection of a very small concentration (5.12 mu g Cg(-1) soil) of C-13-labeled glycine (C-13(2), 99 atom %) to soils in situ. Plots were treated with elevated temperature (+1 degrees C, T), summer drought (D) and elevated atmospheric carbon dioxide (510 ppm [CO2]), as well as combined treatments (TD, TCO2, DCO2 and TDCO2). The C-13 enrichment of respired CO2 and of phospholipid fatty acids (PLFAs) was determined after 24 h. C-13-glycine incorporation into the biomarker PLFAs for specific microbial groups (Gram positive bacteria, Gram negative bacteria, actinobacteria and fungi) was quantified using gas chromatography-combustion-stable isotope ratio mass spectrometry (GC-C-IRMS). Gram positive bacteria opportunistically utilized the freshly added glycine substrate, i.e. incorporated C-13 in all treatments, whereas fungi had minor or no glycine derived C-13-enrichment, hence slowly reacting to a new substrate. The effects of elevated CO2 did suggest increased direct incorporation of glycine in microbial biomass, in particular in G(+) bacteria, in an ecosystem subjected to elevated CO2. Warming decreased the concentration of PLFAs in general. The FACE CO2 was C-13-depleted (delta C-13 = 12.2 parts per thousand) compared to ambient (delta C-13=similar to-8 parts per thousand), and this enabled observation of the integrated longer term responses of soil microorganisms to the FACE over one year. All together, the bacterial (and not fungal) utilization of glycine indicates substrate preference and resource partitioning in the microbial community, and therefore suggests a diversified response pattern to future changes in substrate availability and climatic factors.

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