To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

A quick and robust method… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

A quick and robust method for quantification of the hypersensitive response in plants

Journal article
Authors Oskar N. Johansson
Anders K. Nilsson
Mikael Gustavsson
Thomas Backhaus
Mats X. Andersson
Mats Ellerström
Published in Peerj
Volume 3
ISSN 2167-8359
Publication year 2015
Published at Department of Biological and Environmental Sciences
Language en
Links dx.doi.org/10.7717/peerj.1469
Keywords Hypersensitive response (HR), Programmed cell death (PCD), Electrolyte leakage, Effector-triggered-, PATHOGEN PSEUDOMONAS-SYRINGAE, DISEASE RESISTANCE GENE, ARABIDOPSIS-THALIANA, CELL-DEATH, ERWINIA-AMYLOVORA, DEFENSE, ACID, TOBACCO, GALACTOLIPIDS, ACCUMULATION
Subject categories Botany

Abstract

One of the most studied defense reactions of plants against microbial pathogens is the hypersensitive response (HR). The HR is a complex multicellular process that involves programmed cell death at the site of infection. A standard method to quantify plant defense and the HR is to measure the release of cellular electrolytes into water after infiltration with pathogenic bacteria. In this type of experiment, the bacteria are typically delivered into the plant tissue through syringe infiltration. Here we report the development of a vacuum infiltration protocol that allows multiple plant lines to be infiltrated simultaneously and assayed for defense responses. Vacuum infiltration did not induce more wounding response in Arabidopsis leaf tissue than syringe inoculation, whereas throughput and reproducibility were improved. The method was used to study HR-induced electrolyte loss after treatment with the bacterium Pseudomonas syringae pv. tomato DC3000 harboring the effector AvrRpm1, AvrRpt2 or AvrRps4. Specifically, the influence of bacterial titer on AvrRpm1-induced HR was investigated. Not only the amplitude, but also the timing of the maximum rate of the HR reaction was found to be dose-dependent. Finally, using vacuum infiltration, we were able quantify induction of phospholipase D activity after AvrRpm1 recognition in leaves labeled with (PO4)-P-33.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?