To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Antiplasmodial and cytoto… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Antiplasmodial and cytotoxic activities of the constituents of Turraea robusta and Turraea nilotica

Journal article
Authors Beatrice N. Irungu
N. Adipo
J. A. Orwa
F. Kimani
M. Heydenreich
J. O. Midiwo
Per Martin Björemark
Mikael Håkansson
A. Yenesew
Mate Erdelyi
Published in Journal of Ethnopharmacology
Volume 174
Pages 419-425
ISSN 0378-8741
Publisher Elsevier Ireland Ltd
Publication year 2015
Published at Department of Chemistry and Molecular Biology
Pages 419-425
Language en
Links dx.doi.org/10.1016/j.jep.2015.08.03...
Keywords Antiplasmodial activity, Azadironolide, Cytotoxicity, Limonoid, Toonacilin, Toonapubesins F, Turraea nilotica, Turraea robusta, 11 epi toonacillin, 12alpha acetoxy 7 deacetylazadirone, 1alpha,3alpha diacety 7alpha tigloyvillasinin, antimalarial agent, azadirone, chloroquine, glucoside, hispidol B, hypoxanthine, mzikonone, niloticin, phytosterol, piscidinol A, plant extract, plant medicinal product, protolimonoid, sitosterol 3 o beta dextro glucopyranoside, stigmasterol, stigmasterol 3 o beta dextro glucopyranoside acetate, toonapubesin F, Turraea nilotica extract, Turraea robusta extract, turranolide, unclassified drug, acetylation, animal cell, antimalarial activity, Article, bark, breast cancer, controlled study, HEp 2 cell line, human, human cell, larynx carcinoma, medicinal plant, mouse, nonhuman, Plasmodium falciparum, Vero cell line, X ray crystallography, X ray diffraction
Subject categories Biochemistry and Molecular Biology, Pharmacology

Abstract

Ethnopharmacological relevance: Turraea robusta and Turraea nilotica are African medicinal plants used for the treatment of a wide variety of diseases, including malaria. The genus Turraea is rich in limonoids and other triterpenoids known to possess various biological activities. Materials and methods: From the stem bark of T. robusta six compounds, and from various parts of T. nilotica eleven compounds were isolated by the use of a combination of chromatographic techniques. The structures of the isolated compounds were elucidated using NMR and MS, whilst the relative configuration of one of the isolated compounds, toonapubesin F, was established by X-ray crystallography. The antiplasmodial activities of the crude extracts and the isolated constituents against the D6 and W2 strains of Plasmodium falciparum were determined using the semiautomated micro dilution technique that measures the ability of the extracts to inhibit the incorporation of (G-3H, where G is guanine) hypoxanthine into the malaria parasite. The cytotoxicity of the crude extracts and their isolated constituents was evaluated against the mammalian cell lines African monkey kidney (vero), mouse breast cancer (4T1) and human larynx carcinoma (HEp2). Results: The extracts showed good to moderate antiplasmodial activities, where the extract of the stem bark of T. robusta was also cytotoxic against the 4T1 and the HEp2 cells (IC50<10 μg/ml). The compounds isolated from these extracts were characterized as limonoids, protolimonoids and phytosterol glucosides. These compounds showed good to moderate activities with the most active one being azadironolide, IC50 2.4±0.03 μM and 1.1±0.01 μM against the D6 and W2 strains of Plasmodium falciparum, respectively; all other compounds possessed IC50 14.4-40.5 μM. None of the compounds showed significant cytotoxicity against vero cells, yet four of them were toxic against the 4T1 and HEp2 cancer cell lines with piscidinol A having IC50 8.0±0.03 and 8.4 ±0.01 μM against the 4T1 and HEp2 cells, respectively. Diacetylation of piscidinol A resulted in reduced cytotoxicity. Conclusion: From the medicinal plants T. robusta and T. nilotica, twelve compounds were isolated and characterized; two of the isolated compounds, namely 11-epi-toonacilin and azadironolide showed good antiplasmodial activity with the highest selectivity indices. © 2015 The Authors. Published by Elsevier Ireland Ltd.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?