To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Structural features of th… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Structural features of the O-antigen of Rickettsia typhi, the etiological agent of endemic typhus

Journal article
Authors M. Peturova
Varvara Vitiazeva
R. Toman
Published in Acta Virologica
Volume 59
Issue 3
Pages 228-233
ISSN 0001-723X
Publication year 2015
Published at Institute of Biomedicine
Pages 228-233
Language en
Links dx.doi.org/10.4149/av_2015_03_228
Keywords Rickettsia typhi, lipopolysaccharide, O-antigen, chemical composition, structure, weil-felix test, murine typhus, lipopolysaccharide, monosaccharides, similarity, Virology
Subject categories Virology

Abstract

Elucidation of the O-specific polysaccharide chain of lipopolysaccharide (LPS) from Rickettsia typhi, the etiological agent of endemic typhus, is described. Structural information was established by a combination of monosaccharide and methylation analyses of the O-chain, and by mass (MS) and nuclear magnetic resonance (NMR) spectrometries of oligosaccharides arised through its hydrofluoric (HF) acid degradation. Based on the combined data from these experiments, two major polymer populations of the O-specific chain have been determined with the following structural features: alpha-L-QuiNAc-(1 -> 4)-[alpha-D-Glc-(1 -> 3)-alpha-L-QuiNAc-(1 -> 4)](n) -alpha-D-Glc-(1 -> 4)-alpha-D-Glc ->,alpha-D-Glc-(1 -> 3)-alpha-L-QuiNAc-(1 -> 4)-[alpha-D-Glc-(1 -> 3)-alpha-L-QuiNAc-(1 -> 4)](n)-alpha-D-Glc ->. The linear backbone is most probably flanked with short side chains of D-GlcNAc-(1 -> 3)-alpha-L-QuiNAc-(1 -> 3)-D-GlcNAc -> that are attached to it via L-QuiNAc as a branching point. It is suggested that a dimer alpha-L-QuiNAc-(1 -> 3)-alpha-D-GlcNAc may represent a common epitope in the O-antigens of Proteus vulgaris OX19 and R. typhi responsible for the observed serological cross-reactivity.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?