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Over-Expression Analysis of All Eight Subunits of the Molecular Chaperone CCT in Mammalian Cells Reveals a Novel Function for CCTdelta

Journal article
Authors M. Spiess
Meriem Echbarthi
Andreas Svanstrom
R. Karlsson
Julie Grantham
Published in Journal of Molecular Biology
Volume 427
Issue 17
Pages 2757-2764
ISSN 0022-2836
Publication year 2015
Published at Department of Chemistry and Molecular Biology
Pages 2757-2764
Language en
Links dx.doi.org/10.1016/j.jmb.2015.06.00...
Keywords chaperonin, structured illumination microscopy, spatial organization, plasma membrane, ATPase, saccharomyces-cerevisiae, cytosolic chaperonin, actin, proteins, binding, mechanism, oligomers, cycle
Subject categories Biochemistry and Molecular Biology

Abstract

Chaperonin containing tailless complex polypeptide 1 (CCT) forms a classical chaperonin barrel structure where two rings of subunits surround a central cavity. Each ring consists of eight distinct subunits, creating a complex binding interface that makes CCT unique among the chaperonins. In addition to acting as a multimeric chaperonin, there is increasing evidence indicating that the CCT subunits, when monomeric, possess additional functions. Here we assess the role of the CCT subunits individually, using a GFP (green fluorescent protein) tagging approach to express each of the subunits in their monomeric form in cultured mammalian cells. Over-expression of CCTdelta, but not the other seven CCT subunits, results in the appearance of numerous protrusions at the cell surface. Two point mutations, one in the apical domain and one in the ATP binding pocket of CCTdelta, that abolish protrusion formation have been identified, consistent with the apical domain containing a novel interaction site that is influenced by the ATPase activity in the equatorial domain. Structured illumination microscopy, together with sub-cellular fractionation, reveals that only the wild-type CCTdelta is associated with the plasma membrane, thus connecting spatial organization with surface protrusion formation. Expression of the equivalent subunit in yeast, GFP-Cct4, rescues growth of the temperature-sensitive strain cct4-1 at the non-permissive temperature, indicative of conserved subunit-specific activities for CCTdelta. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-ncV4.0/).

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