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Binding Affinity, Specificity and Comparative Biodistribution of the Parental Murine Monoclonal Antibody MX35 (Anti-NaPi2b) and Its Humanized Version Rebmab200.

Journal article
Authors Sture Lindegren
Luciana N S Andrade
Tom Bäck
Camila Maria L Machado
Bruno Brasil Horta
Carlos Buchpiguel
Ana Maria Moro
Oswaldo Keith Okamoto
Lars Jacobsson
Elin Cederkrantz
Kohshin Washiyama
Emma Aneheim
Stig Palm
Holger Jensen
Maria Carolina B Tuma
Roger Chammas
Ragnar Hultborn
Per Albertsson
Published in PloS one
Volume 10
Issue 5
Pages e0126298
ISSN 1932-6203
Publication year 2015
Published at Institute of Clinical Sciences, Department of Radiation Physics
Institute of Clinical Sciences, Department of Oncology
Pages e0126298
Language en
Links dx.doi.org/10.1371/journal.pone.012...
Subject categories Cancer and Oncology, Radiological physics

Abstract

The aim of this preclinical study was to evaluate the characteristics of the monoclonal antibody Rebmab200, which is a humanized version of the ovarian-specific murine antibody MX35. This investigation contributes to the foundation for future clinical α-radioimmunotherapy of minimal residual ovarian cancer with 211At-Rebmab200. Here, the biodistribution of 211At-Rebmab200 was evaluated, as was the utility of 99mTc-Rebmab200 for bioimaging. Rebmab200 was directly compared with its murine counterpart MX35 in terms of its in-vitro capacity for binding the immobilized NaPi2B epitope and live cells; we also assessed its biodistribution in nude mice carrying subcutaneous OVCAR-3 tumors. Tumor antigen and cell binding were similar between Rebmab200 and murine MX35, as was biodistribution, including normal tissue uptake and in-vivo tumor binding. We also demonstrated that 99mTc-Rebmab200 can be used for single-photon emission computed tomography of subcutaneous ovarian carcinomas in tumor-bearing mice. Taken together, our data support the further development of Rebmab200 for radioimmunotherapy and diagnostics.

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