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Optical mapping of single DNA molecules in nanochannels: A novel method for identification and characterization of antibiotic resistance

Conference paper
Authors Lena Nyberg
Gustav Emilsson
A. Nilsson
E. Lagerstedt
C. Noble
Liselott Svensson-Stadler
Nahid Karami
Fei Sjöberg
Edward R.B. Moore
Joachim Fritzsche
Erik Kristiansson
T. Ambjörnsson
Fredrik Westerlund
Published in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Pages 1045-1047
ISBN 978-09-79-80647-6
Publication year 2015
Published at Institute of Neuroscience and Physiology
Institute of Biomedicine, Department of Infectious Medicine
Pages 1045-1047
Language en
Keywords Antibiotic resistance; Nanofluidics; Optical mapping; Plasmid DNA
Subject categories Biochemistry and Molecular Biology

Abstract

The use, and overuse, of antibiotics has during the last decade led to a dramatic increase in antibiotic resistance and there is a crying need for novel methods for fast identification of antibiotic resistance genes. We here demonstrate how our previously developed assay for optical mapping of DNA in nanochannels can be used for characterization of resistance genes located on plasmid DNA from bacteria. The assay requires extremely small sample volumes and does neither rely on PCR, nor culturing of bacteria, which greatly reduces the time for analysis.

Page Manager: Webmaster|Last update: 9/11/2012
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