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Atlantic Salmon Carries a Range of Novel O-Glycan Structures Differentially Localized on Skin and Intestinal Mucins

Journal article
Authors Chunsheng S. Jin
János T Padra
Kristina Sundell
Henrik Sundh
Niclas G. Karlsson
Sara K. Lindén
Published in Journal of Proteome Research
Volume 14
Issue 8
Pages 3239-3251
ISSN 1535-3893
Publication year 2015
Published at Department of Biological and Environmental Sciences
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 3239-3251
Language en
Links dx.doi.org/10.1021/acs.jproteome.5b...
Keywords Atlantic salmon, fish, O-glycan, mucins, skin, gastrointestinal tract, mucus, glycosylation, RAINBOW-TROUT, OLIGOSACCHARIDE-ALDITOLS, MISGURNUS-ANGUILLICAUDATUS, ONCORHYNCHUS-MYKISS, JELLY COATS, MUC2 MUCIN, ZEBRAFISH, GLYCOPROTEIN, GLYCOSYLATION, CHAINS, Biochemical Research Methods
Subject categories Biochemistry

Abstract

Aquaculture is a growing industry, increasing the need for understanding host-pathogen interactions in fish. The skin and mucosal surfaces, covered by a mucus layer composed of mucins, is the first point of contact between fish and pathogens. Highly O-glycosylated mucins have been shown to be an important part of the defense against pathogens, and pathogens bind to host surfaces using lectin-like adhesins. However, knowledge of piscine O-glycosylation is very limited. We characterized mucin O-glycosylation of five freshwater acclimated Atlantic salmon, using mass spectrometry. Of the 109 O-glycans found, most were sialylated and differed in distribution among skin, pyloric ceca, and proximal and distal intestine. Skin O-glycans were shorter (2-6 residues) and less diverse (33 structures) than intestinal O-glycans (2-13 residues, 93 structures). Skin mucins carried O-glycan cores 1, 2, 3, and 5 and three types of sialic acids (Neu5Ac, Neu5Gc, and Kdn) and had sialyl-Tn as the predominant structure. Intestinal mucins carried only cores 1, 2, and 5, Neu5Ac was the only sialic acid present, and sialylated core S was the most dominant structure. This structural characterization can be used for identifying structures of putative importance in host-pathogen interactions for further testing in biological assays and disease intervention therapies.

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