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Round robin test on quantification of amyloid-β 1-42 in cerebrospinal fluid by mass spectrometry.

Journal article
Authors Josef Pannee
Johan Gobom
Leslie M Shaw
Magdalena Korecka
Erin E Chambers
Mary Lame
Rand Jenkins
William Mylott
Maria C Carrillo
Ingrid Zegers
Henrik Zetterberg
Kaj Blennow
Erik Portelius
Published in Alzheimer's & dementia : the journal of the Alzheimer's Association
Volume 12
Issue 1
Pages 55–59
ISSN 1552-5279
Publication year 2016
Published at Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Pages 55–59
Language en
Keywords Amyloid beta; Cerebrospinal fluid; Mass spectrometry; Interlaboratory study
Subject categories Neurochemistry


Cerebrospinal fluid (CSF) amyloid-β 1-42 (Aβ42) is an important biomarker for Alzheimer's disease, both in diagnostics and to monitor disease-modifying therapies. However, there is a great need for standardization of methods used for quantification. To overcome problems associated with immunoassays, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has emerged as a critical orthogonal alternative.

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