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Circulating gonadotropins and ovarian adiponectin system are modulated by acupuncture independently of sex steroid or beta-adrenergic action in a female hyperandrogenic rat model of polycystic ovary syndrome

Journal article
Authors Manuel Maliqueo
Anna Benrick
Asif Alvi
Julia Johansson
Miao Sun
F. Labrie
Claes Ohlsson
Elisabet Stener-Victorin
Published in Molecular and Cellular Endocrinology
Volume 412
Issue C
Pages 159-169
ISSN 0303-7207
Publication year 2015
Published at Institute of Neuroscience and Physiology
Institute of Neuroscience and Physiology, Department of Physiology
Centre for Bone and Arthritis Research
Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
Pages 159-169
Language en
Links dx.doi.org/10.1016/j.mce.2015.04.02...
Keywords Polycystic ovary syndrome, Animal model, Acupuncture, Estradiol, Sympathetic nervous system, RANDOMIZED CONTROLLED-TRIAL, LOW-FREQUENCY ELECTROACUPUNCTURE, MESSENGER-RIBONUCLEIC-ACID, SYMPATHETIC-NERVE ACTIVITY, BONE-MINERAL, DENSITY, OVARIECTOMIZED RATS, PHYSICAL-EXERCISE, GENE-EXPRESSION, CORTICAL BONE, GROWTH-FACTOR, Cell Biology, Endocrinology & Metabolism
Subject categories Endocrinology and Diabetes, Endocrinology

Abstract

Acupuncture with combined manual and low-frequency electrical stimulation, or electroacupuncture (EA), reduces endocrine and reproductive dysfunction in women with polycystic ovary syndrome (PCOS), likely by modulating sympathetic nerve activity or sex steroid synthesis. To test this hypothesis, we induced PCOS in rats by prepubertal implantation of continuous-release letrozole pellets (200 mu g/day) or vehicle. Six weeks later, rats were treated for 5-6 weeks with low-frequency EA 5 days/week, subcutaneous injection of 17 beta-estradiol (2.0 mu g) every fourth day, or a beta-adrenergic blocker (propranolol hydrochloride, 0.1 mg/kg) 5 days/week. Letrozole controls were handled without needle insertion or injected with sesame oil every fourth day. Estrous cyclicity, ovarian morphology, sex steroids, gonadotropins, insulin-like growth factor I, bone mineral density, and gene and protein expression in ovarian tissue were measured. Low-frequency EA induced estrous-cycle changes, decreased high levels of circulating luteinizing hormone (LH) and the LH/follicle-stimulating hormone (FSH) ratio, decreased high ovarian gene expression of adiponectin receptor 2, and increased expression of adiponectin receptor 2 protein and phosphorylation of ERK1/2. EA also increased cortical bone mineral density. Propranolol decreased ovarian expression of Foxo3, Srd5al, and Hif1a. Estradiol decreased circulating LH, induced estrous cycle changes, and decreased ovarian expression of Adipor1, Foxo3, and Pik3r1. Further, total bone mineral density was higher in the letrozole-estradiol group. Thus, EA modulates the circulating gonadotropin levels independently of sex steroids or beta-adrenergic action and affects the expression of ovarian adiponectin system. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

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