To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Imaging Mass Spectrometry… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Imaging Mass Spectrometry for Single-Cell Analysis

Chapter in book
Authors Nhu TN Phan
John S. Fletcher
Andrew G Ewing
Published in Reference Module in Chemistry, Molecular Sciences and Chemical Engineering
ISBN 978-0-12-409547-2
Publisher Elsevier
Publication year 2014
Published at Department of Chemistry and Molecular Biology
Language en
Links dx.doi.org/10.1016/B978-0-12-409547...
Subject categories Analytical Chemistry

Abstract

Single-cell imaging is a very important area in biological, medical, and pharmaceutical research, yet it is one of the most challenging fields for mass spectrometry. Imaging mass spectrometry (IMS), which is a powerful label-free analytical technique, has proved its capability to chemically visualize single cells with high sensitivity, chemical selectivity, and in certain cases subcellular spatial resolution. We present here an overview of the capabilities and current progress of IMS, particularly secondary ion mass spectrometry, matrix-assisted laser desorption ionization, and desorption electrospray ionization for single-cell imaging. The principles and technical developments of each technique are introduced. Critical aspects regarding single-cell imaging are addressed, especially sensitivity, spatial resolution, and sample handling. Current achievements in this field of application are also presented for both 2-D imaging and 3-D imaging. Furthermore, we address the potential contribution of IMS single-cell ‘omics’ and discuss its future development.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?