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In vivo application of tissue-engineered veins using autologous peripheral whole blood: A proof of concept study

Journal article
Authors Michael Olausson
Vijay Kumar Kuna
G. Travnikova
H. Bäckdahl
Pradeep B Patil
Robert Saalman
Helena Borg
Anders Jeppsson
Suchitra Sumitran-Holgersson
Published in EBioMedicine
Volume 1
Issue 1
Pages 72-79
ISSN 2352-3964
Publication year 2014
Published at Institute of Clinical Sciences, Department of Surgery
Institute of Clinical Sciences, Department of Pediatrics
Institute of Medicine, Department of Molecular and Clinical Medicine
Pages 72-79
Language en
Links dx.doi.org/10.1016/j.ebiom.2014.09....
Keywords Endothelial precursors, Tissue-engineering, Vascular diseases, Vein conduits
Subject categories Surgery

Abstract

Vascular diseases are increasing health problems affecting >25 million individuals in westernized societies. Such patients could benefit fromtransplantation of tissue-engineered vascular grafts using autologous cells. One challenge that has limited this development is the need for cell isolation, and risks associated with ex vivo expanded stem cells. Herewe demonstrate a novel approach to generate transplantable vascular grafts using decellularized allogeneic vascular scaffolds, repopulatedwith peripheralwhole blood (PWB) in vitro in a bioreactor. Circulating, VEGFR-2+/CD45+ and a smaller fraction of VEGFR-2+/CD14+ cells contributed to repopulation of the graft. SEMmicrographs showed flat cells on the luminal surface of the grafts consistentwith endothelial cells. For clinical validation, two autologous PWBtissue-engineered vein conduits were prepared and successfully used for bypass procedures in two pediatric patients. These results provide a proof of principle for the generation of transplantable vascular grafts using a simple autologous blood sample, making it clinically feasible globally.

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