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Rapid 3D fluorescence imaging of individual optically trapped living immune cells

Journal article
Authors D. Wolfson
M. Steck
Martin Persson
G. McNerney
A. Popovich
Mattias Goksör
T. Huser
Published in Journal of Biophotonics
Volume 8
Issue 3
Pages 208-216
ISSN 1864-0648
Publication year 2015
Published at Department of Physics (GU)
Pages 208-216
Language en
Links dx.doi.org/10.1002/jbio.201300153
Keywords 3-D imaging, Confocal microscopy, Fluorescence microscopy, Jurkat cells, Optical tweezers
Subject categories Physical Sciences

Abstract

We demonstrate an approach to rapidly characterize living suspension cells in 4 dimensions while they are immobilized and manipulated within optical traps. A single, high numerical aperture objective lens is used to separate the imaging plane from the trapping plane. This facilitates full control over the position and orientation of multiple trapped cells using a spatial light modulator, including directed motion and object rotation, while also allowing rapid 4D imaging. This system is particularly useful in the handling and investigation of the behavior of non-adherent immune cells. We demonstrate these capabilities by imaging and manipulating living, fluorescently stained Jurkat T cells.

Page Manager: Webmaster|Last update: 9/11/2012
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