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Studies of mucus in mouse stomach, small intestine, and colon. II. Gastrointestinal mucus proteome reveals Muc2 and Muc5ac accompanied by a set of core proteins.

Journal article
Authors Ana María Rodríguez-Piñeiro
Joakim H. Bergström
Anna Ermund
Jenny K Gustafsson
André Schütte
Malin E V Johansson
Gunnar C. Hansson
Published in American journal of physiology. Gastrointestinal and liver physiology
Volume 305
Issue 5
Pages G348-56
ISSN 1522-1547
Publication year 2013
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages G348-56
Language en
Links dx.doi.org/10.1152/ajpgi.00047.2013
Keywords Animals, Biotinylation, Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Cluster Analysis, Colon, metabolism, Female, Gastric Mucosa, metabolism, Intestinal Mucosa, metabolism, Intestine, Small, metabolism, Male, Mice, Mice, Inbred C57BL, Mucin 5AC, metabolism, Mucin-2, metabolism, Mucus, metabolism, Proteomics, methods, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry
Subject categories Cell and Molecular Biology

Abstract

The mucus that protects the surface of the gastrointestinal tract is rich in specialized O-glycoproteins called mucins, but little is known about other mucus proteins or their variability along the gastrointestinal tract. To ensure that only mucus was analyzed, we combined collection from explant tissues mounted in perfusion chambers, liquid sample preparation, single-shot mass spectrometry, and specific bioinformatics tools, to characterize the proteome of the murine mucus from stomach to distal colon. With our approach, we identified ∼1,300 proteins in the mucus. We found no differences in the protein composition or abundance between sexes, but there were clear differences in mucus along the tract. Noticeably, mucus from duodenum showed similarities to the stomach, probably reflecting the normal distal transport. Qualitatively, there were, however, fewer differences than might had been anticipated, suggesting a relatively stable core proteome (∼80% of the total proteins identified). Quantitatively, we found significant differences (∼40% of the proteins) that could reflect mucus specialization throughout the gastrointestinal tract. Hierarchical clustering pinpointed a number of such proteins that correlated with Muc2 (e.g., Clca1, Zg16, Klk1). This study provides a deeper knowledge of the gastrointestinal mucus proteome that will be important in further understanding this poorly studied mucosal protection system.

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