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A high-throughput LC-MS/MS assay for quantification of artesunate and its metabolite dihydroartemisinin in human plasma and saliva.

Journal article
Authors Sofia Birgersson
Therese Ericsson
Antje Blank
Cornelia von Hagens
Michael Ashton
Kurt-Jürgen Hoffmann
Published in Bioanalysis
Volume 6
Issue 18
Pages 2357-69
ISSN 1757-6180
Publication year 2014
Published at Institute of Neuroscience and Physiology, Department of Pharmacology
Pages 2357-69
Language en
Links dx.doi.org/10.4155/bio.14.116
Subject categories Pharmacology

Abstract

AIM: Saliva is an alternative sampling matrix to plasma, offering a noninvasive technique, but requires a highly sensitive bioanalytical method. MATERIALS & METHODS: An API 3000 triple quadrupole mass spectrometer with an electrospray ionization source operated in the positive ion mode was used for the analysis. RESULTS: A high-throughput LC-MS/MS method using SPE for the quantification of artesunate and dihydroartemisinin in plasma and saliva has been optimized and validated according to US FDA guidelines. For both analytes the LLOQ was determined to 5 ng/ml and the calibration range was 5-1000 ng/ml for artesunate and 5-2000 ng/ml for dihydroartemisinin. CONCLUSION: For the first time, a bioanalytical method for determination of artesunate and dihydroartemisinin in human saliva has been described, showing possible applicability in clinical saliva samples in addition to plasma samples.

Page Manager: Webmaster|Last update: 9/11/2012
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