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Identifying bacteria using DNA binding maps

Conference paper
Authors Gustav Emilsson
A. Nilsson
Lena Nyberg
C. Noble
L. Svensson Stadler
Edward R.B. Moore
T. Ambjörnsson
J.O. Tegenfeldt
Fredrik Westerlund
Published in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013
Volume 1
Pages 473-475
ISBN 978-163266624-6
Publication year 2014
Published at
Pages 473-475
Language en
Keywords Bacterial identification; Nanofluidics; Optical mapping
Subject categories Physical Chemistry, Nano Technology

Abstract

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Page Manager: Webmaster|Last update: 9/11/2012
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