To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Allosteric regulation of … - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Allosteric regulation of phosphofructokinase controls the emergence of glycolytic oscillations in isolated yeast cells

Journal article
Authors Anna-Karin Gustavsson
David D. van Niekerk
Caroline B. Adiels
Bob Kooi
Mattias Goksör
Jacky L. Snoep
Published in The FEBS Journal
Volume 281
Issue 12
Pages 2784-2793
ISSN 1742-464X
Publication year 2014
Published at Department of Physics (GU)
Pages 2784-2793
Language en
Keywords cell heterogeneity, glycolysis, limit cycle oscillation, mathematical model, microfluidic chamber
Subject categories Physical Sciences, Optics, Biochemistry, Bioinformatics and Systems Biology


Oscillations are widely distributed in nature and synchronization of oscillators has been described at the cellular level (e.g. heart cells) and at the population level (e.g. fireflies). Yeast glycolysis is the best known oscillatory system, although it has been studied almost exclusively at the population level (i.e. limited to observations of average behaviour in synchronized cultures). We studied individual yeast cells that were positioned with optical tweezers in a microfluidic chamber to determine the precise conditions for autonomous glycolytic oscillations. Hopf bifurcation points were determined experimentally in individual cells as a function of glucose and cyanide concentrations. The experiments were analyzed in a detailed mathematical model and could be interpreted in terms of an oscillatory manifold in a three-dimensional state-space; crossing the boundaries of the manifold coincides with the onset of oscillations and positioning along the longitudinal axis of the volume sets the period. The oscillatory manifold could be approximated by allosteric control values of phosphofructokinase for ATP and AMP.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?