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Improving multiphoton microscopy using annular beam shaping, focusing on imaging of human skin

Conference paper
Authors Johan Borglin
N.J. Durr
Stina Guldbrand
O. Ferhanoǧlu
Ann-Marie Wennberg
Dag Hanstorp
A. Ben-Yakar
Marica B Ericson
Published in Multiphoton Microscopy in the Biomedical Sciences XIV: 2-4 February 2014, San Francisco, California, United States. Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume 8948
ISSN 1605-7422
Publication year 2014
Published at Department of Chemistry and Molecular Biology
Department of Physics (GU)
Language en
Keywords annular beams , Bessel beams , Multiphoton miocroscopy , two photon microscopy
Subject categories Optics


Multiphoton fluorescence microscopy (MPM) is a method for high resolution, non-invasive investigations of biological tissue. The aim of introducing an annular shaped laser beam is to reduce the ouf-of-focus generated background signal improving imaging of light scattering tissue such as human skin. Simulations show that 50% of the beam radius can be blocked, while preserving the shape of the point spread function. Initial experiments performed on a phantom consisting of fluorescein and fluorescent beads embedded in agar by using a custom built MPM-set up show that by introducing a simple beam blocker to create an annular beam, the background signal is reduced with approximately 5%. Future work will include optimizing the set up, and creating phantoms with more light scattering properties. © 2014 SPIE.

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