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Loss of Ubp3 increases Silencing, decreases Unequal Recombination in rDNA, and shortens the Replicative Life Span in Saccharomyces cerevisiae.

Journal article
Authors David Öling
Rehan Masoom
Kristian Kvint
Published in Molecular Biology of the Cell
Volume 25
Issue 12
Pages 1916-1924
ISSN 1059-1524
Publication year 2014
Published at Department of Chemistry and Molecular Biology
Pages 1916-1924
Language en
Links dx.doi.org/10.1091/mbc.E13-10-0591
https://gup.ub.gu.se/file/134700
Subject categories Molecular biology, Microbiology

Abstract

Ubp3 is a conserved ubiquitin protease that acts as an antisilencing factor in MAT and telomeric regions. Here we show that ubp3∆ mutants also display increased silencing in ribosomal DNA (rDNA). Consistent with this, RNA polymerase II occupancy is lower in cells lacking Ubp3 than in wild-type cells in all heterochromatic regions. Moreover, in a ubp3∆ mutant, unequal recombination in rDNA is highly suppressed. We present genetic evidence that this effect on rDNA recombination, but not silencing, is entirely dependent on the silencing factor Sir2. Further, ubp3∆ sir2∆ mutants age prematurely at the same rate as sir2∆ mutants. Thus our data suggest that recombination negatively influences replicative life span more so than silencing. However, in ubp3∆ mutants, recombination is not a prerequisite for aging, since cells lacking Ubp3 have a shorter life span than isogenic wild-type cells. We discuss the data in view of different models on how silencing and unequal recombination affect replicative life span and the role of Ubp3 in these processes.

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