To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Characterization of brown… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Characterization of brown adipose tissue in the human perirenal depot.

Journal article
Authors Per-Arne Svensson
Kristin Lindberg
Jenny M Hoffmann
Magdalena Taube
Maria J Pereira
Tala Mohsen-Kanson
Anne-Laure Hafner
Magnus Rizell
Jenny Palming
Christian Dani
Maria K Svensson
Published in Obesity
Volume 22
Issue 8
Pages 1830–1837
ISSN 1930-7381
Publication year 2014
Published at Institute of Medicine, Department of Molecular and Clinical Medicine
Pages 1830–1837
Language en
Links dx.doi.org/10.1002/oby.20765
Subject categories Surgery

Abstract

Objective To characterize brown adipose tissue (BAT) in the human perirenal adipose tissue depot. Method Perirenal adipose tissue biopsies were obtained from 55 healthy kidney donors. Expression analysis was performed using microarray, real-time PCR, immunoblotting and immunohistochemistry. Additional studies using human stem cells were performed. Results UCP1 gene expression analysis revealed a large intra-individual variation in the perirenal adipose tissue biopsies. Both multi- and unilocular UCP1-positive adipocytes were detected in several of the adipose tissue samples analyzed by immunohistochemical staining. Microarray analysis identified 54 genes that were overexpressed in UCP1-positive perirenal adipose tissue. Real-time PCR analysis of BAT candidate genes revealed a set of genes that were highly correlated to UCP1 and a set of three transcription factor genes (PRDM16, PGC1α, and RXRγ) that were highly correlated to each other. RXRγ displayed nuclear immunoreactivity in brown adipocytes and an increased gene expression during brown adipogenesis in human stem cells. Conclusion Our data provides the first molecular characterization of BAT in the perirenal adipose tissue depot. Furthermore, it highlights the transcription factor RXRγ as a new player in BAT development.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?