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Role of Androgen and Estrogen Receptors for the Action of Dehydroepiandrosterone (DHEA)

Journal article
Authors Cecilia Engdahl
Marie K Lagerquist
Alexandra Stubelius
Annica Andersson
Erik Studer
Claes Ohlsson
Lars Westberg
Hans Carlsten
Helena Forsblad d'Elia
Published in Endocrinology
Volume 155
Issue 3
Pages 889-896
ISSN 0013-7227
Publication year 2014
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Centre for Bone and Arthritis Research
Institute of Neuroscience and Physiology, Department of Pharmacology
Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
Pages 889-896
Language en
Links dx.doi.org/10.1210/en.2013-1561
https://gup.ub.gu.se/file/127115
Keywords PRIMARY SJOGRENS-SYNDROME, SUBMANDIBULAR-GLANDS, MALE-MICE, CORTICAL, BONE, ACTIVATION, CELL, HORMONE, BINDING, PROTEIN, ALPHA
Subject categories Endocrinology

Abstract

Dehydroepiandrosterone (DHEA) is an abundant steroid hormone, and its mechanism of action is yet to be determined. The aim of this study was to elucidate the importance of androgen receptors (ARs) and estrogen receptors (ERs) for DHEA function. Orchidectomized C57BL/6 mice were treated with DHEA, DHT, 17 beta-estradiol-3-benzoate (E2), or vehicle. Orchidectomized AR-deficient (ARKO) mice and wild-type (WT) littermates were treated with DHEA or vehicle for 2.5 weeks. At termination, bone mineral density (BMD) was evaluated, thymus and seminal vesicles were weighted, and submandibular glands (SMGs) were histologically examined. To evaluate the in vivo ER activation of the classical estrogen signaling pathway, estrogen response element reporter mice were treated with DHEA, DHT, E2, or vehicle, and a reporter gene was investigated in different sex steroid-sensitive organs after 24 hours. DHEA treatment increased trabecular BMD and thymic atrophy in both WT and ARKO mice. In WT mice, DHEA induced enlargement of glands in the SMGs, whereas this effect was absent in ARKO mice. Furthermore, DHEA was able to induce activation of classical estrogen signaling in bone, thymus, and seminal vesicles but not in the SMGs. In summary, the DHEA effects on trabecular BMD and thymus do not require signaling via AR and DHEA can activate the classical estrogen signaling in these organs. In contrast, DHEA induction of gland size in the SMGs is dependent on AR and does not involve classical estrogen signaling. Thus, both ERs and ARs are involved in mediating the effects of DHEA in an organ-dependent manner.

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