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Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery.

Journal article
Authors Cecilia Geijer
Dagmara Medrala Klein
Elzbieta Petelenz-Kurdziel
Abraham Ericsson
Maria Smedh
Mikael Andersson
Mattias Goksör
Mariona Nadal-Ribelles
Francesc Posas
Marcus Krantz
Bodil Nordlander
Stefan Hohmann
Published in The FEBS journal
Volume 280
Issue 16
Pages 3854-67
ISSN 1742-4658
Publication year 2013
Published at Department of Chemistry and Molecular Biology
Department of Physics (GU)
Core Facilities, Centre for Cellular Imaging
Pages 3854-67
Language en
Links dx.doi.org/10.1111/febs.12382
Keywords Adaptation, Physiological, Cell Nucleus, metabolism, Glycerol, adverse effects, Glycerol-3-Phosphate Dehydrogenase (NAD+), genetics, metabolism, Hypertonic Solutions, Indicators and Reagents, adverse effects, Kinetics, MAP Kinase Signaling System, Mitogen-Activated Protein Kinases, genetics, metabolism, Mutation, Osmotic Pressure, Phosphorylation, Promoter Regions, Genetic, Protein Processing, Post-Translational, Protein Transport, Saccharomyces cerevisiae, genetics, metabolism, Saccharomyces cerevisiae Proteins, genetics, metabolism, Stress, Physiological, Transcription, Genetic, Up-Regulation
Subject categories Biological Sciences

Abstract

The control of activity and localization of transcription factors is critical for appropriate transcriptional responses. In eukaryotes, signal transduction components such as mitogen-activated protein kinase (MAPK) shuttle into the nucleus to activate transcription. It is not known in detail how different amounts of nuclear MAPK over time affect the transcriptional response. In the present study, we aimed to address this issue by studying the high osmolarity glycerol (HOG) system in Saccharomyces cerevisiae. We employed a conditional osmotic system, which changes the period of the MAPK Hog1 signal independent of the initial stress level. We determined the dynamics of the Hog1 nuclear localization and cell volume by single-cell analysis in well-controlled microfluidics systems and compared the responses with the global transcriptional output of cell populations. We discovered that the onset of the initial transcriptional response correlates with the potential of cells for rapid adaptation; cells that are capable of recovering quickly initiate the transcriptional responses immediately, whereas cells that require longer time to adapt also respond later. This is reflected by Hog1 nuclear localization, Hog1 promoter association and the transcriptional response, but not Hog1 phosphorylation, suggesting that a presently uncharacterized rapid adaptive mechanism precedes the Hog1 nuclear response. Furthermore, we found that the period of Hog1 nuclear residence affects the amplitude of the transcriptional response rather than the spectrum of responsive genes.

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