To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

MTERF1 Binds mtDNA to Pre… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

MTERF1 Binds mtDNA to Prevent Transcriptional Interference at the Light-Strand Promoter but Is Dispensable for rRNA Gene Transcription Regulation

Journal article
Authors M. Terzioglu
B. Ruzzenente
J. Harmel
A. Mourier
Elisabeth Jemt
Marcela Davila Lopez
C. Kukat
J. B. Stewart
R. Wibom
C. Meharg
B. Habermann
Maria Falkenberg
Claes M Gustafsson
C. B. Park
N. G. Larsson
Published in Cell Metabolism
Volume 17
Issue 4
Pages 618-626
ISSN 1550-4131
Publication year 2013
Published at Institute of Biomedicine
Pages 618-626
Language en
Links dx.doi.org/10.1016/j.cmet.2013.03.0...
Keywords MAMMALIAN MITOCHONDRIAL RIBOSOME, TERMINATION FACTOR MTERF, IN-VITRO, OXIDATIVE-PHOSPHORYLATION, PROTEIN COMPLEX, DNA, POLYMERASE, REVEALS, MICE, TRANSLATION, RISTIANSON TW
Subject categories Basic Medicine

Abstract

Mitochondrial transcription termination factor 1, MTERF1, has been reported to couple rRNA gene transcription initiation with termination and is therefore thought to be a key regulator of mammalian mitochondrial ribosome biogenesis. The prevailing model is based on a series of observations published over the last two decades, but no in vivo evidence exists to show that MTERF1 regulates transcription of the heavy-strand region of mtDNA containing the rRNA genes. Here, we demonstrate that knockout of Mterf1 in mice has no effect on mitochondrial rRNA levels or mitochondrial translation. Instead, loss of Mterf1 influences transcription initiation at the light-strand promoter, resulting in a decrease of de novo transcription manifested as reduced 7S RNA levels. Based on these observations, we suggest that MTERF1 does not regulate heavy-strand transcription, but rather acts to block transcription on the opposite strand of mtDNA to prevent transcription interference at the light-strand promoter.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?