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Molecular Characterization of the alpha-Subunit of Na+/K+ ATPase from the Euryhaline Barnacle Balanus improvisus Reveals Multiple Genes and Differential Expression of Alternative Splice Variants

Journal article
Authors Ulrika Lind
Magnus Alm Rosenblad
Anna-Lisa Wrange
Kristina Sundell
Per R. Jonsson
Carl André
Jonathan N. Havenhand
Anders Blomberg
Published in Plos One
Volume 8
Issue 10
Pages artikel nr e77069
ISSN 1932-6203
Publication year 2013
Published at Department of Biological and Environmental Sciences
Department of Chemistry and Molecular Biology
Pages artikel nr e77069
Language en
Links dx.doi.org/10.1371/journal.pone.007...
Keywords SALMON SALMO-SALAR, TROPONIN-T GENE, NA-K-ATPASE, ATLANTIC SALMON, H+-ATPASE, BALANOMORPH BARNACLES, ARTEMIA-FRANCISCANA, SALINITY, TOLERANCE, NA++K+-ATPASE, NA,K-ATPASE, AN PM, 2011, MOL CELL PROTEOMICS, V10, GREGORIS TB, 2009, BMC MOL BIOL, V10, STER BA, 1970, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-, KEYASU K, 1990, AMERICAN JOURNAL OF PHYSIOLOGY, V259, PC619, ESSLEY TA, 1992, AMERICAN JOURNAL OF PHYSIOLOGY, V262, PC743
Subject categories Biological Sciences

Abstract

The euryhaline bay barnacle Balanus improvisus has one of the broadest salinity tolerances of any barnacle species. It is able to complete its life cycle in salinities close to freshwater (3 PSU) up to fully marine conditions (35 PSU) and is regarded as one of few truly brackish-water species. Na+/K+ ATPase (NAK) has been shown to be important for osmoregulation when marine organisms are challenged by changing salinities, and we therefore cloned and examined the expression of different NAKs from B. improvisus. We found two main gene variants, NAK1 and NAK2, which were approximately 70% identical at the protein level. The NAK1 mRNA existed in a long and short variant with the encoded proteins differing only by 27 N-terminal amino acids. This N-terminal stretch was coded for by a separate exon, and the two variants of NAK1 mRNAs appeared to be created by alternative splicing. We furthermore showed that the two NAK1 isoforms were differentially expressed in different life stages and in various tissues of adult barnacle, i.e the long isoform was predominant in cyprids and in adult cirri. In barnacle cyprid larvae that were exposed to a combination of different salinities and pCO(2) levels, the expression of the long NAK1 mRNA increased relative to the short in low salinities. We suggest that the alternatively spliced long variant of the Nak1 protein might be of importance for osmoregulation in B. improvisus in low salinity conditions.

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