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Signal Recognition Particle RNA in Dinoflagellates and the Perkinsid Perkinsus marinus

Journal article
Authors H. Zhang
D. A. Campbell
N. R. Sturm
Magnus Alm Rosenblad
C. F. Dungan
S. J. Lin
Published in Protist
Volume 164
Issue 5
Pages 748-761
ISSN 1434-4610
Publication year 2013
Published at Department of Chemistry and Molecular Biology
Pages 748-761
Language en
Links dx.doi.org/10.1016/j.protis.2013.07...
Keywords Dinoflagellates, Perkinsus marinus, SRP RNA, protein translocation, SPLICED LEADER RNA, TRYPANOSOMA-BRUCEI, ENDOPLASMIC-RETICULUM, PROTEIN, TRANSLOCATION, ALU-DOMAIN, GENE, IDENTIFICATION, PURIFICATION, PHYLOGENIES, SEQUENCES, CHAELI S, 1992, MOLECULAR AND BIOCHEMICAL PARASITOLOGY, V51, P55
Subject categories Biological Sciences

Abstract

In dinoflagellates and perkinsids, the molecular structure of the protein translocating machinery is unclear. Here, we identified several types of full-length signal recognition particle (SRP) RNA genes from Karenia brevis (dinoflagellate) and Perkinsus marinus (perkinsid). We also identified the four SRP S-domain proteins, but not the two Alu domain proteins, from P. marinus and several dinoflagellates. We mapped both ends of SRP RNA transcripts from K. brevis and P. marinus, and obtained the 3' end from four other dinoflagellates. The lengths of SRP RNA are predicted to be similar to 260-300 nt in dinoflagellates and 280-285 nt in P. marinus. Although these SRP RNA sequences are substantially variable, the predicted structures are similar. The genomic organization of the SRP RNA gene differs among species. In K. brevis, this gene is located downstream of the spliced leader (SL) RNA, either as SL RNA-SRP RNA-tRNA gene tandem repeats, or within a SL RNA-SRP RNA-tRNA-U6-5S rRNA gene cluster. In other dinoflagellates, SRP RNA does not cluster with SL RNA or 5S rRNA genes. The majority of P. marinus SRP RNA genes array as tandem repeats without the above-mentioned small RNA genes. Our results capture a snapshot of a potentially complex evolutionary history of SRP RNA in alveolates. (c) 2013 Elsevier GmbH. All rights reserved.

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