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Comparison of Dermatophyte PCR Kit with Conventional Methods for Detection of Dermatophytes in Skin Specimens.

Journal article
Authors Nahid Kondori
Parisa Afshari Tehrani
Louise Strömbeck
Jan Faergemann
Published in Mycopathologia
Volume 176
Issue 3-4
Pages 237-241
ISSN 1573-0832
Publication year 2013
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 237-241
Language en
Links dx.doi.org/10.1007/s11046-013-9691-...
Keywords Dermatophyte PCR, Dermatophytosis, Skin, Fungal infection
Subject categories Dermatology and Venereal Diseases

Abstract

The laboratory diagnosis of dermatophytosis is usually based on direct microscopic examination and culturing of clinical specimens. A commercial polymerase chain reaction kit (Dermatophyte PCR) has had favorable results when used for detection of dermatophytes and identification of Trichophyton rubrum in nail specimens. This study investigated the efficacy of the Dermatophyte PCR kit for detecting dermatophytosis in 191 hair or skin specimens from patients with suspected dermatophytosis. PCR was positive for 37 % of samples, whereas 31 and 39 % of the specimens were positive by culturing and direct microscopy, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value for PCR analysis were 83, 84, 71, and 91 %, respectively. The sensitivity of the PCR test was higher in specimens obtained from skin (88 %) than in those obtained from hair (58 %), while the specificity remained almost the same (84 and 86 % for skin and hair, respectively). Our results show that the Dermatophyte PCR kit is a promising diagnostic tool for detection of dermatophytosis in skin samples, providing clinicians with a rapid diagnosis.

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