To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Studies of mucus in mouse… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Studies of mucus in mouse stomach, small intestine, and colon. III. Gastrointestinal Muc5ac and Muc2 mucin O-glycan patterns reveal a regiospecific distribution

Journal article
Authors Jessica Holmén Larsson
Kristina A Thomsson
Ana María Rodríguez-Piñeiro
Hasse Karlsson
Gunnar C. Hansson
Published in American Journal of Physiology-Gastrointestinal and Liver Physiology
Volume 305
Issue 5
Pages G357-G363
ISSN 0193-1857
Publication year 2013
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages G357-G363
Language en
Links dx.doi.org/10.1152/ajpgi.00048.2013
Keywords glycomics, Mucin, Muc2, Muc5ac, mass spectrometry, oligosaccharide, glycan, BLOOD-GROUP-A, LINKED OLIGOSACCHARIDES, NIPPOSTRONGYLUS-BRASILIENSIS, HELICOBACTER-PYLORI, GUT MICROBIOTA, GLYCOSYLATION, MICE, GLYCOSYLTRANSFERASE, DIVERSITY, BACTERIA, ATES OF AMERICA, Animals, Chromatography, Liquid, Colon, metabolism, Electrophoresis, Polyacrylamide Gel, Female, Fucose, metabolism, Gastric Mucosa, metabolism, Glycosylation, Intestinal Mucosa, metabolism, Intestine, Small, metabolism, Male, Mass Spectrometry, Mice, Mice, Inbred C57BL, Mucin 5AC, metabolism, Mucin-2, metabolism, Mucus, metabolism, N-Acetylneuraminic Acid, metabolism, Proteomics, methods, Sulfates, metabolism
Subject categories Clinical Medicine, Health Sciences

Abstract

The mouse intestinal mucus is mainly made up by the gel-forming Muc2 mucin and the stomach surface mucus Muc5ac, both extensively O-glycosylated. The oligosaccharide diversity provides a vast library of potential recognition sites for both commensal and pathogenic organisms. The mucin glycans are thus likely very important for the selection and maintenance of a stable intestinal flora. Here we have explored the O-glycan patterns of the mouse gastrointestinal tract mucins. The mucins from the mucus of the distal and proximal colon, ileum, jejunum, duodenum, and stomach of conventionally raised wild-type (C57BL/6) mice were separated by composite gel electrophoresis. The O-linked glycans were released by reductive elimination and structurally characterized by liquid chromatography-mass spectrometry. The mucins glycans were mostly core 2 type [Gal beta 1-3(GlcNAc beta 1-6)GalNAcol], but also core 1 (Gal beta 1-3GalNAcol). In the stomach about half of the Muc5ac mucin O-glycans were neutral and many monosulfated, but with a low grade of sialylation and fucosylation. Mouse ileum, jejunum, and duodenum had similar glycan patterns dominated by sialylated and sulfated core 2 glycans, but few fucosylated. Colon was on the other hand dominated by highly charged fucosylated glycans. The distal colon is different from the proximal colon because different biosynthetic pathways are utilized, although sialylated and sulfated glycans were highly abundant in both parts. The sulfation was higher in the distal colon, whereas sialic acid was more common in the proximal colon. Many fucosylated glycans were found in both the proximal and distal colon. Thus the mucin O-glycans vary along the mouse gastrointestinal tract.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?