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Zinc Finger Protein 148 Is Dispensable for Primitive and Definitive Hematopoiesis in Mice

Journal article
Authors Anna Nilton
Volkan I. Sayin
Anna Staffas
Erik Larsson
Julia Rolf
Marleen MR Petit
Lars Palmqvist
Birgitta Swolin
Susanna Cardell
Per Lindahl
Published in Plos One
Volume 8
Issue 7
Pages e70022
ISSN 1932-6203
Publication year 2013
Published at Wallenberg Laboratory
Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
Institute of Biomedicine, Department of Microbiology and Immunology
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Institute of Medicine, Department of Molecular and Clinical Medicine
Pages e70022
Language en
Links dx.doi.org/10.1371/journal.pone.007...
Keywords TRANSCRIPTION FACTOR, GENE-EXPRESSION, ZBP-89, CELL, PROMOTER, MUSCLE, BINDS, BOX, embryology, DNA-Binding Proteins, Hematopoiesis, Mice, Knockout,embryology, growth & development, embryology, growth & development, Transcription Factors, deficiency, genetics
Subject categories Molecular medicine (genetics and pathology), Medical cell biology

Abstract

Hematopoiesis is regulated by transcription factors that induce cell fate and differentiation in hematopoietic stem cells into fully differentiated hematopoietic cell types. The transcription factor zinc finger protein 148 (Zfp148) interacts with the hematopoietic transcription factor Gata1 and has been implicated to play an important role in primitive and definitive hematopoiesis in zebra fish and mouse chimeras. We have recently created a gene-trap knockout mouse model deficient for Zfp148, opening up for analyses of hematopoiesis in a conventional loss-of-function model in vivo. Here, we show that Zfp148-deficient neonatal and adult mice have normal or slightly increased levels of hemoglobin, hematocrit, platelets and white blood cells, compared to wild type controls. Hematopoietic lineages in bone marrow, thymus and spleen from Zfp148(gt/gt) mice were further investigated by flow cytometry. There were no differences in T-cells (CD4 and CD8 single positive cells, CD4 and CD8 double negative/positive cells) in either organ. However, the fraction of CD69- and B220-positive cells among lymphocytes in spleen was slightly lower at postnatal day 14 in Zfp148(gt/gt) mice compared to wild type mice. Our results demonstrate that Zfp148-deficient mice generate normal mature hematopoietic populations thus challenging earlier studies indicating that Zfp148 plays a critical role during hematopoietic development.

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