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Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH.

Journal article
Authors Malin E V Johansson
Gunnar C. Hansson
Published in Methods in molecular biology (Clifton, N.J.)
Volume 842
Pages 229-35
ISSN 1940-6029
Publication year 2012
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 229-35
Language en
Links dx.doi.org/10.1007/978-1-61779-513-...
Keywords Animals, Bacteria, chemistry, isolation & purification, Immunohistochemistry, In Situ Hybridization, Fluorescence, Mice, Mucins, analysis, chemistry, Mucus, chemistry, Tissue Fixation
Subject categories Basic Medicine

Abstract

As mucus is highly hydrated, special care has to be taken to preserve this in histological preparations during immunostaining. Here, we describe how to fix tissues in such a way that the mucus is preserved in paraffin-embedded tissue. We also describe how the major macromolecular components in the mucus, the mucins, are immunostained and how bacteria can be localized in preparations with preserved mucus by fluorescent in situ hybridization.

Page Manager: Webmaster|Last update: 9/11/2012
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