To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Characterization of Human… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Characterization of Human Thymic Exosomes

Journal article
Authors Gabriel Skogberg
Judith Gudmundsdottir
Sjoerd van der Post
Kerstin Sandström
S. Bruhn
M. Benson
L. Mincheva-Nilsson
V. Baranov
Esbjörn Telemo
Olov Ekwall
Published in Plos One
Volume 8
Issue 7
ISSN 1932-6203
Publication year 2013
Published at Institute of Medicine, Department of Rheumatology and Inflammation Research
Core Facilities, Proteomics
Institute of Clinical Sciences, Department of Pediatrics
Language en
Links dx.doi.org/10.1371/journal.pone.006...
https://gup.ub.gu.se/file/113872
Keywords promiscuous gene-expression, mhc class-ii, epithelial-cells, dendritic, cells, antigen presentation, t-cells, self-antigen, tolerance, proteins, vesicles
Subject categories Immunogenetics, Clinical immunology

Abstract

Exosomes are nanosized membrane-bound vesicles that are released by various cell types and are capable of carrying proteins, lipids and RNAs which can be delivered to recipient cells. Exosomes play a role in intercellular communication and have been described to mediate immunologic information. In this article we report the first isolation and characterization of exosomes from human thymic tissue. Using electron microscopy, particle size determination, density gradient measurement, flow cytometry, proteomic analysis and microRNA profiling we describe the morphology, size, density, protein composition and microRNA content of human thymic exosomes. The thymic exosomes share characteristics with previously described exosomes such as antigen presentation molecules, but they also exhibit thymus specific features regarding surface markers, protein content and microRNA profile. Interestingly, thymic exosomes carry proteins that have a tissue restricted expression in the periphery which may suggest a role in T cell selection and the induction of central tolerance. We speculate that thymic exosomes may provide the means for intercellular information exchange necessary for negative selection and regulatory T cell formation of the developing thymocytes within the human thymic medulla.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?