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Comparisons of Immunoassay and Mass Spectrometry Measurements of Serum Estradiol Levels and Their Influence on Clinical Association Studies in Men

Journal article
Authors Claes Ohlsson
Maria E. Nilsson
Åsa Tivesten
Henrik Ryberg
Dan Mellström
M. K. Karlsson
O. Ljunggren
F. Labrie
E. S. Orwoll
D. M. Lee
S. R. Pye
T. W. O'Neill
J. D. Finn
J. E. Adams
K. A. Ward
S. Boonen
G. Bartfai
F. F. Casanueva
G. Forti
A. Giwercman
T. S. Han
I. T. Huhtaniemi
K. Kula
M. E. J. Lean
N. Pendleton
M. Punab
D. Vanderschueren
F. C. W. Wu
Liesbeth Vandenput
Published in Journal of Clinical Endocrinology & Metabolism
Volume 98
Issue 6
Pages E1097-E1102
ISSN 0021-972X
Publication year 2013
Published at Wallenberg Laboratory
Institute of Neuroscience and Physiology
Centre for Bone and Arthritis Research
Institute of Medicine
Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
Pages E1097-E1102
Language en
Keywords peripheral arterial-disease, reactive protein-levels, middle-aged men, elderly-men, sex-hormones, testosterone, risk, sweden, assays, mros
Subject categories Clinical Medicine, Endocrinology


Context: Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known Objective: Our objective was to compare immunoassay and MS measurements of E2 levels in men and Design and Setting: Middle-aged and older male subjects participating in the population-based Main Outcome Measures: Immunoassay and MS measurements of serum E2 were compared and Results: Within each cohort, serum E2 levels obtained by immunoassay and MS correlated moderately Conclusions: Our findings suggest interference in the immunoassay E2 analyses, possibly by CRP or a

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