To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Top-down approach to stud… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Top-down approach to studying biological components using ToF-SIMS

Journal article
Authors Alan M. Piwowar
John S. Fletcher
Nicholas P. Lockyer
John C. Vickerman
Published in Surface and Interface Analysis
Volume 43
Issue 1-2
Pages 265–268
ISSN 0142-2421
Publication year 2011
Published at
Pages 265–268
Language en
Subject categories Analytical Chemistry, Cell Biology

Abstract

We present in this article a novel approach to identifying subcellular components from spectral images collected with ToF-SIMS. The method utilizes a separation technique to isolate and enrich cellular fractions which are then examined with ToF-SIMS to identify peak or peak ratio markers to assign reference spectra. The ultimate aim of the project is to utilize these reference spectra to identify cellular components from spectral images. We present data from three such extracts (nuclei, cytosol and membrane-bound organelles) which confirms that characteristic spectral differences do exist between these systems. These results indicate that such a ‘top-down’ approach to subcellular analysis can aid cellular imaging with ToF-SIMS.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?