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ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems

Journal article
Authors Helen L. Kotze
Emily G. Armitage
John S. Fletcher
Alex Henderson
Kaye J. Williams
Nicholas P. Lockyer
John C. Vickerman
Published in Surface and Interface Analysis
Volume 45
Issue 1
Pages 277–281
ISSN 0142-2421
Publication year 2013
Published at
Pages 277–281
Language en
Subject categories Analytical Chemistry, Bioinformatics and Systems Biology

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance.

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