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Stenotrophomonas interspecies differentiation and identification by gyrB sequence analysis.

Journal article
Authors Liselott Svensson-Stadler
Sashka A Mihaylova
Edward R.B. Moore
Published in FEMS microbiology letters
Volume 327
Issue 1
Pages 15-24
ISSN 1574-6968
Publication year 2012
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 15-24
Language en
Links dx.doi.org/10.1111/j.1574-6968.2011...
Keywords Bacterial Proteins, genetics, Bacterial Typing Techniques, methods, DNA Gyrase, genetics, Environmental Microbiology, Gram-Negative Bacterial Infections, microbiology, Molecular Sequence Data, Phylogeny, Plants, microbiology, Stenotrophomonas, classification, enzymology, genetics, isolation & purification
Subject categories Microbiology, Clinical Laboratory Medicine, Biomedical Laboratory Science/Technology

Abstract

Stenotrophomonas species are found commonly in environmental and clinical samples; Stenotrophomonas maltophilia is an important opportunistic pathogen of humans. Traditional phenotyping protocols, as well as genotyping by 16S rRNA gene sequence analysis, do not reliably distinguish the species of Stenotrophomonas. Sequence analyses of two targeted PCR-amplified regions of the gyrB gene, which encodes the β-subunit of DNA gyrase, enabled resolution and identification of these species. Most type strains of the different species of Stenotrophomonas exhibited more than 7% dissimilarity in the gyrB gene sequences. Among these, strains identified as the same species exhibited sequence dissimilarities up to 4.6% and 5.9% for the two regions, respectively. Strains identified as S. maltophilia, with 16S rRNA gene sequence similarities > 99.0%, were grouped within a 'S. maltophilia complex'; these organisms exhibited gyrB similarities as low as 93%. Many of these strains possessed genomic DNA similarities with the type strain of S. maltophilia CCUG 5866(T) below 70%. These data, including gyrB sequence comparisons, indicate that strains identified as S. maltophilia may comprise distinct, new species.

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