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Strain-level typing and identification of bacteria using mass spectrometry-based proteomics.

Journal article
Authors Roger Karlsson
Max Davidson
Liselott Svensson-Stadler
Anders Karlsson
Kenneth Olesen
Elisabet Carlsohn
Edward R.B. Moore
Published in Journal of proteome research
Volume 11
Issue 5
Pages 2710-20
ISSN 1535-3907
Publication year 2012
Published at Institute of Biomedicine
Department of Chemistry and Molecular Biology
Institute of Biomedicine, Department of Infectious Medicine
Pages 2710-20
Language en
Links dx.doi.org/10.1021/pr2010633
Keywords Amino Acid Sequence, Bacterial Proteins, analysis, chemistry, Bacterial Typing Techniques, methods, Cell Membrane, chemistry, Genome, Bacterial, Helicobacter pylori, chemistry, classification, genetics, Lipids, chemistry, Mass Spectrometry, methods, Membrane Proteins, analysis, chemistry, Molecular Sequence Data, Multilocus Sequence Typing, Peptide Fragments, analysis, chemistry, Phylogeny, Proteolysis, Proteomics, instrumentation, methods, Reproducibility of Results, Species Specificity
Subject categories Clinical Medicine, Clinical Laboratory Medicine

Abstract

Because of the alarming expansion in the diversity and occurrence of bacteria displaying virulence and resistance to antimicrobial agents, it is increasingly important to be able to detect these microorganisms and to differentiate and identify closely related species, as well as different strains of a given species. In this study, a mass spectrometry proteomics approach is applied, exploiting lipid-based protein immobilization (LPI), wherein intact bacterial cells are bound, via membrane-gold interactions, within a FlowCell. The bound cells are subjected to enzymatic digestion for the generation of peptides, which are subsequently identified, using LC-MS. Following database matching, strain-specific peptides are used for subspecies-level discrimination. The method is shown to enable a reliable typing and identification of closely related strains of the same bacterial species, herein illustrated for Helicobacter pylori .

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