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Rapid discrimination of the causal agents of urinary tract infection using ToF-SIMS with chemometric cluster analysis

Conference paper
Authors John S. Fletcher
A. Henderson
R. M. Jarvis
N. P. Lockyer
J. C. Vickerman
R. Goodacre
Published in Applied Surface Science
Volume 252
Issue 19
Pages 6869-6874
ISSN 0169-4332
Publication year 2006
Published at
Pages 6869-6874
Language en
Subject categories Analytical Chemistry

Abstract

Advances in time of flight secondary ion mass spectrometry (ToF-SIMS) have enabled this technique to become a powerful tool for the analysis of biological samples. Such samples are often very complex and as a result full interpretation of the acquired data can be extremely difficult. To simplify the interpretation of these information rich data, the use of chemometric techniques is becoming widespread in the ToF-SIMS community. Here we discuss the application of principal components-discriminant function analysis (PC-DFA) to the separation and classification of a number of bacterial samples that are known to be major causal agents of urinary tract infection. A large data set has been generated using three biological replicates of each isolate and three machine replicates were acquired from each biological replicate. Ordination plots generated using the PC-DFA are presented demonstrating strain level discrimination of the bacteria. The results are discussed in terms of biological differences between certain species and with reference to FT-IR, Raman spectroscopy and pyrolysis mass spectrometric studies of similar samples. (c) 2006 Elsevier B.V. All rights reserved.

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