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Redefinition of the carbohydrate binding specificity of Helicobacter pylori BabA adhesin.

Journal article
Authors John Benktander
Jonas Ångström
Michael Breimer
Susann Teneberg
Published in The Journal of biological chemistry
Volume 287
Issue 38
Pages 31712-31724
ISSN 1083-351X
Publication year 2012
Published at Institute of Biomedicine
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 31712-31724
Language en
Links dx.doi.org/10.1074/jbc.M112.387654
Subject categories Surgery

Abstract

Certain Helicobacter pylori strains adhere to the human gastric epithelium using the BabA adhesin (blood group antigen binding adhesin). All BabA-expressing H. pylori strains bind to the blood group O determinants on type 1 core chains, i.e. to the Lewis b antigen (Fucα2Galβ3(Fucα4)GlcNAc; Leb) and the H type 1 determinant (Fucα2Galβ3GlcNAc). Recently BabA strains have been categorized into those recognizing only Leb and H type 1 determinants (designated specialist strains), and those that also bind to A and B type 1 determinants (designated generalist strain). Here, the structural requirements for carbohydrate recognition by generalist and specialist BabA were further explored by binding of these types of strains to a panel of different glycosphingolipids. Three glycosphingolipids recognized by both specialist and generalist BabA were isolated from the small intestine of a blood group O pig, and characterized by mass spectrometry and proton NMR as H type 1 pentaglycosylceramide (Fucα2Galβ3GlcNAcβ3Galβ4Glcβ1Cer), Globo H hexaglycosylceramide (Fucα2Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), and a mixture of three complex glycosphingolipids (Fucα2Galβ4GlcNAcβ6(Fucα2Galβ3GlcNAcβ3)Galβ3GlcNAcβ3Galβ4Glcβ1Cer, Fucα2Galβ3GlcNAcβ6(Fucα2Galβ3GlcNAcβ3)Galβ3GlcNAcβ3Galβ4Glcβ1Cer and Fucα2Galβ4(Fucα3)GlcNAcβ6(Fucα2Galβ3GlcNAcβ3)Galβ3GlcNAcβ3Galβ4Glcβ1Cer). In addition to the binding of both strains to the Globo H hexaglycosylceramide, i.e. a blood group O determinant on a type 4 core chain, the generalist strain bound to the Globo A heptaglycosylceramide (GalNAcα3(Fucα2)Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), i.e. a blood group A determinant on a type 4 core chain. The binding of BabA to the two sets of isoreceptors is due to onformational similarities of the terminal disaccharides of H type 1 and Globo H, and of the terminal trisaccharides of A type 1 and Globo A.

Page Manager: Webmaster|Last update: 9/11/2012
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