To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Sustained glycolytic osci… - University of Gothenburg, Sweden Till startsida
To content Read more about how we use cookies on

Sustained glycolytic oscillations in individual isolated yeast cells

Journal article
Authors Anna-Karin Gustavsson
D. D. van Niekerk
Caroline B. Adiels
F. B. du Preez
Mattias Goksör
J. L. Snoep
Published in Febs Journal
Volume 279
Issue 16
Pages 2837-2847
ISSN 1742-464X
Publication year 2012
Published at Department of Physics (GU)
Pages 2837-2847
Language en
Keywords cell heterogeneity, glycolysis, limit-cycle oscillation, mathematical model, microfluidic chamber, saccharomyces-cerevisiae, nutrient starvation, optical tweezers, single-cell, make-up, synchronization, dynamics, manipulation, laser, acetaldehyde
Subject categories Biochemistry, Physical Sciences


Yeast glycolytic oscillations have been studied since the 1950s in cell-free extracts and intact cells. For intact cells, sustained oscillations have so far only been observed at the population level, i.e. for synchronized cultures at high biomass concentrations. Using optical tweezers to position yeast cells in a microfluidic chamber, we were able to observe sustained oscillations in individual isolated cells. Using a detailed kinetic model for the cellular reactions, we simulated the heterogeneity in the response of the individual cells, assuming small differences in a single internal parameter. This is the first time that sustained limit-cycle oscillations have been demonstrated in isolated yeast cells. Database ?The mathematical model described here has been submitted to the JWS Online Cellular Systems Modelling Database and can be accessed at free of charge.

Page Manager: Webmaster|Last update: 9/11/2012

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?