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The Littorina Sequence Database (LSD) – an online resource for genomic data.

Journal article
Authors Björn Canbäck
Carl André
Juan Galindo
Kerstin Johannesson
Tomas Johansson
Marina Panova
Anders Tunlid
Roger Butlin
Published in Molecular Ecology Resources
Volume 12
Issue 1
Pages 142-148
ISSN 1755-0998
Publication year 2012
Published at Linnaeus Centre for Marine Evolutionary Biology (CEMEB)
Department of Biological and Environmental Sciences
Pages 142-148
Language en
Links dx.doi.org/10.1111/j.1755-0998.2011...
Keywords ecotype; EST database; Littorina saxatilis; model organism; speciation
Subject categories Biological Sciences

Abstract

We present an interactive, searchable expressed sequence tag database for the periwinkle snail Littorina saxatilis, an upcoming model species in evolutionary biology. The database is the result of a hybrid assembly between Sanger and 454 sequences, 1290 and 147 491 sequences respectively. Normalized and non-normalized cDNA was obtained from different ecotypes of L. saxatilis collected in the UK and Sweden. The Littorina sequence database (LSD) contains 26 537 different contigs, of which 2453 showed similarity with annotated proteins in UniProt. Querying the LSD permits the selection of the taxonomic origin of blast hits for each contig, and the search can be restricted to particular taxonomic groups. The database allows access to UniProt annotations, blast output, protein family domains (PFAM) and Gene Ontology. The database will allow users to search for genetic markers and identifying candidate genes or genes for expression analyses. It is open for additional deposition of sequence information for L. saxatilis and other species of the genus Littorina. The LSD is available at http://mbio-serv2.mbioekol.lu.se/Littorina/

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