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Analysis of assembly of secreted mucins

Journal article
Authors Malin E V Johansson
Gunnar C. Hansson
Published in Methods in Molecular Biology
Volume 842
Pages 109-121
ISSN 1064-3745
Publication year 2012
Published at Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 109-121
Language en
Links dx.doi.org/10.1007/978-1-61779-513-...
Keywords Mucus, Mucin, Glycan, Intestine, Pulse chase, Agarose-polyacrylamide gel electrophoresis
Subject categories Basic Medicine

Abstract

Studies of assembly and secretion of gel-forming mucins are complex. The pulse-chase methods for mucins described here include metabolic radiolabeling and labeling in animals with azido-GalNAc. The labeled mucins are analyzed by composite agarose-polyacrylamide gel electrophoresis and autoradiography or by mucus-preserving tissue fixation and Click-iT® chemistry.

Page Manager: Webmaster|Last update: 9/11/2012
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