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In vitro characterization and osteoblast responses to nanostructured photocatalytic TiO2 coated surfaces.

Journal article
Authors Mariko Hayashi
Ryo Jimbo
Liselott Lindh
Javier Sotres
Takashi Sawase
Kamal Mustafa
Martin Andersson
Ann Wennerberg
Published in Acta biomaterialia
Volume 8
Issue 6
Pages 2411-6
ISSN 1878-7568
Publication year 2012
Published at
Pages 2411-6
Language en
Keywords Photocatalytic properties; Hydrophilicity; Osteoblasts; Nanostructures; Anatase
Subject categories Biomaterials Science

Abstract

The aims of the study were to characterize a nanostructured photoactive titanium dioxide (TiO(2)) coating and to compare the cellular response of human osteoblasts before and after ultraviolet (UV) irradiation of the coating. A specific nanostructured TiO(2) powder (Degussa P-25), which consists of approximately 80% anatase and 20% rutile, was spin-coated onto commercially pure titanium discs, and was heat-treated thereafter. After topographical, chemical and photocatalytic property characterizations, human osteoblasts were cultured on the coated discs before and after UV irradiation. Cell morphology was evaluated by scanning electron microscopy (SEM), and cell viability was analysed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT) assay. From the contact angle analysis, the wettability significantly improved after UV irradiation. The cultured cells were flattened with numerous elongated lammellipodia; however, no morphological differences were indicated between -UV and +UV surfaces. The MTT assay analysis showed that -UV surface presented significantly higher viability compared to the +UV surface except for one cell population group at 3h where there were no differences. The nanostructured photoactive TiO(2) surface improved its hydrophilicity by UV irradiation, however no enhancing effect in cell response was confirmed at the time tested compared to the non-irradiated surface.

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