To the top

Page Manager: Webmaster
Last update: 9/11/2012 3:13 PM

Tell a friend about this page
Print version

Lactobacillus and Bifidob… - University of Gothenburg, Sweden Till startsida
Sitemap
To content Read more about how we use cookies on gu.se

Lactobacillus and Bifidobacterium species do not secrete protease that cleaves the MUC2 mucin which organises the colon mucus.

Journal article
Authors Durai B Subramani
Malin E V Johansson
Gunnar Dahlén
Gunnar C. Hansson
Published in Beneficial microbes
Volume 1
Issue 4
Pages 343-50
ISSN 1876-2891
Publication year 2010
Published at Institute of Odontology
Institute of Biomedicine
Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
Pages 343-50
Language en
Links dx.doi.org/10.3920/BM2010.0039
Keywords Animals, Bacterial Proteins, genetics, metabolism, Bifidobacterium, enzymology, genetics, isolation & purification, Cell Line, Colon, metabolism, microbiology, Extracellular Space, enzymology, genetics, Humans, Intestinal Mucosa, metabolism, microbiology, Lactobacillus, enzymology, genetics, isolation & purification, Mice, Mucin-2, chemistry, genetics, metabolism, Peptide Hydrolases, genetics, metabolism, Protein Processing, Post-Translational, Protein Structure, Tertiary
Subject categories Physiology, Microbiology in the medical area

Abstract

The colon epithelium is covered by two layers of mucus built around the MUC2 mucin. An inner dense and attached mucus layer does not allow bacteria to penetrate, thus keeping the epithelial cell surface free from bacteria. An outer loose mucus layer is the habitat for the commensal bacterial microbiota. The inner mucus layer is renewed from the epithelial side and gets converted into the outer layer due to proteolytic cleavages by host proteases. We have now analysed if potential probiotic bacteria, namely Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium lactis, can secrete protease that cleaves the MUC2 mucin. We found that none of the potential probiotic bacteria Lactobacillus and Bifidobacterium could cleave the MUC2 core protein in the form of recombinant MUC2 N and C-termini although they secreted active proteases. This was in contrast to crude mixtures of oral and faecal bacteria that cleaved the MUC2 mucin. This observation further supports the view that these potential probiotic bacteria are of no harm to the host, as these bacteria cannot disrupt the mucin organised mucus as long as they are covered by glycans.

Page Manager: Webmaster|Last update: 9/11/2012
Share:

The University of Gothenburg uses cookies to provide you with the best possible user experience. By continuing on this website, you approve of our use of cookies.  What are cookies?