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A metabolomics approach to elucidate effects of food deprivation in juvenile rainbow trout (Oncorhynchus mykiss)

Journal article
Authors Andreas Kullgren
Linda Samuelsson
D. G. Joakim Larsson
Björn Thrandur Björnsson
Elisabeth Jönsson Bergman
Published in American Journal of Physiology-Regulatory Integrative and Comparative Physiology
Volume 299
Issue 6
Pages R1440-R1448
ISSN 0363-6119
Publication year 2010
Published at Institute of Neuroscience and Physiology, Department of Physiology
Department of Zoology, Zoophysiology
Pages R1440-R1448
Language en
Links dx.doi.org/10.1152/ajpregu.00281.20...
Keywords fish, fasting, NMR, lipoprotein, fatty acid, glycogen, protein, amino acid
Subject categories Animal physiology

Abstract

The aim of this study was to evaluate whether NMR-based metabolomics is a suitable method to generate an integrated view on metabolic processes during food deprivation in salmonid fish. Juvenile rainbow trout (Oncorhynchus mykiss) weighing 43-115 g were either fed ad libitum or deprived of food for 28 days at 10 degrees C to investigate catabolic effects on energy reserves and metabolite patterns. The NMR analysis of plasma, liver, and muscle extracts revealed significant fasting-induced changes in the metabolome. Altered plasma lipoprotein levels and tissue-specific patterns of fatty acid mobilization were the most prominent responses, emphasizing the role of lipids as the primary energy source during fasting. In plasma, very-low-density lipoprotein levels increased in food-deprived fish compared with fed fish, whereas levels of high-density lipoprotein decreased. Signs of muscle protein catabolism were also observed as changes in the alanine dynamics. The results further suggest that mechanisms to preserve liver glycogen are present in the food-deprived fish. This study thus demonstrates the utility of NMR-based metabolomics of tissue extracts and plasma to describe the integrated metabolic status of fish.

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