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Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate.

Journal article
Authors Robin Brittain-Long
Johan Westin
Sigvard Olofsson
Magnus Lindh
Lars-Magnus Andersson
Published in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Volume 47
Issue 3
Pages 263-7
ISSN 1873-5967
Publication year 2010
Published at Institute of Biomedicine, Department of Infectious Medicine
Pages 263-7
Language en
Keywords Adult, Aged, Aged, 80 and over, Bacteria, classification, isolation & purification, Bacterial Infections, diagnosis, Community-Acquired Infections, diagnosis, Female, Humans, Male, Middle Aged, Nasopharynx, microbiology, virology, Polymerase Chain Reaction, methods, Prospective Studies, Respiratory Tract Infections, diagnosis, Time Factors, Virus Diseases, diagnosis, Viruses, classification, isolation & purification, Young Adult
Subject categories Infectious Medicine


Nucleic acid amplification techniques have improved the diagnostic possibilities in respiratory tract infections, although their clinical applicability is not yet fully defined. We have evaluated a multiplex real-time PCR method for the detection of 13 respiratory viruses and 2 bacteria (Mycoplasma and Chlamydophila) in a clinical setting.

Page Manager: Webmaster|Last update: 9/11/2012

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