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BAFF, stimulatory DNA and IL-15 stimulates IgA(+) memory B cells and provides a novel approach for analysis of memory responses to mucosal vaccines.

Journal article
Authors Sara Tengvall
Anna Lundgren
Marianne Quiding-Järbrink
Ann-Mari Svennerholm
Published in Vaccine
Volume 28
Issue 33
Pages 5445-50
ISSN 1873-2518
Publication year 2010
Published at Institute of Biomedicine, Department of Microbiology and Immunology
Pages 5445-50
Language en
Links dx.doi.org/10.1016/j.vaccine.2010.0...
Keywords Adjuvants, Immunologic, pharmacology, Adult, Antibodies, Bacterial, blood, immunology, B-Cell Activating Factor, immunology, pharmacology, B-Lymphocytes, immunology, Cholera Vaccines, immunology, pharmacology, DNA, immunology, pharmacology, Female, Humans, Immunity, Mucosal, drug effects, immunology, Immunization, methods, Immunoglobulin A, blood, immunology, Immunologic Memory, drug effects, immunology, Interleukin-15, immunology, pharmacology, Male, Time Factors, Vaccines, Inactivated, immunology, pharmacology
Subject categories Microbiology in the medical area

Abstract

Assessment of immune responses induced by mucosal vaccines is to a large extent based on measurement of IgA levels in mucosal secretions and detection of short-lived effector IgA-secreting cells circulating in peripheral blood. Since these immunological parameters poorly reflect long-term IgA-mediated responses, we sought to investigate novel approaches that would enable detection of vaccine specific IgA memory B cells. We demonstrate that stimulation of human peripheral blood mononuclear cells in vitro with immunostimulatory DNA in combination with B cell-activating factor (BAFF) and IL-15 promotes differentiation of IgA memory B cells to IgA-secreting cells. By using the inactivated oral cholera vaccine Dukoral we demonstrate that vaccine specific IgA memory B cells are induced by oral immunization and are circulating for at least 9 months after vaccination. We also show that stimulated IgA memory B cells do not secrete IgA unless they reencounter the specific antigen.

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